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Biotin is a water soluble vitamin and thus cannot be deposited in the system. Monogastrics produce large quantities of Biotin by bacterial synthesis in the gut, and it was believed that this Biotin together with the vitamin content of the feedstuff covered the requirements of monogastrics.

Although required in smaller quantities than other basal nutrients within diets, deficiencies in minerals (both macro and micro minerals) can cause major metabolic disorder sometimes inductive of mortality and always results in economic losses.